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| Gao,X.Y.; Zhou,X.F.; Wang,H.; Lv,N.; Liu,Y.; Guo,J.R.. |
| This study aimed to investigate the effects of heme oxygenase-1 recombinant Lactococcus lactis (LL-HO-1) on the intestinal barrier of rats with hemorrhagic shock. One hundred Sprague-Dawley male rats (280–320 g) were randomly divided into healthy control group (N group) and hemorrhagic shock group (H group). Each group was subdivided into HO1t, HO2t, HO3t, PBS and LL groups in which rats were intragastrically injected with LL-HO-1 once, twice and three times, PBS and L. lactis (LL), respectively. The mortality, intestinal myeloperoxidase (MPO) activity, intestinal contents of TNF-α, IL-10 and HO-1, and intestinal Chiu's score were determined. Results showed that in N group, the HO-1 content increased after LL-HO-1 treatment, and significant difference was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heme oxygenase-1; Hemorrhagic shock; Lactococcus lactis; Intestinal barrier; Intestinal inflammation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700601 |
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| Nouaille,S.; Bermúdez-Humarán,L.G.; Adel-Patient,K.; Commissaire,J.; Gruss,A.; Wal,J.M.; Azevedo,V.; Langella,P.; Chatel,J.M.. |
| The stabilizing effects of staphylococcal nuclease (Nuc) and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS) on the production of a model food allergen, bovine ß-lactoglobulin (BLG), in Lactococcus lactis were investigated. The fusion of Nuc to BLG (Nuc-BLG) results in higher production and secretion of the hybrid protein. When LEISS was fused to BLG, the production of the resulting protein LEISS-BLG was only slightly improved compared to the one obtained with Nuc-BLG. However, the secretion of LEISS-BLG was dramatically enhanced (~10- and 4-fold higher than BLG and Nuc-BLG, respectively). Finally, the fusion of LEISS to Nuc-BLG resulting in the protein LEISS-Nuc-BLG led to the highest production of the hybrid protein, estimated at ~8 µg/ml... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactococcus lactis; Protein secretion; LEISSTCDA; Staphylococcal nuclease; SS-lactoglobulin; Allergen. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300005 |
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| Tian,Hongtao; Tan,Jianxin; Zhang,Lifang; Gu,Xinxi; Xu,Wentao; Guo,Xinghua; Luo,Yunbo. |
| The effects of the expression of a small heat shock protein (shsp) gene from Streptococcus thermophilus on stress resistance in Lactococcus lactis under different environmental stresses were investigated in this study. pMG36e-shsp, an expression vector, was first constructed by inserting a shsp open reading frame (ORF) cloned from S. thermophilus strain St-QC into pMG36e. Then, a food-grade expression vector, pMG-shsp, was generated by deleting the erythromycin resistance gene from pMG36e-shsp. The transformation rate of pMG-shsp was comparable to that of pMG36e-shsp when each of these two vectors was introduced into L. lactis. These results demonstrated that the shsp ORF could successfully used as a food-grade selection marker in both pMG-shsp and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shsp gene; Streptococcus thermophilus; Lactococcus lactis; Food-grade vector; Stress resistance. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300043 |
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| Freitas,D.A.; Leclerc,S.; Miyoshi,A.; Oliveira,S.C.; Sommer,P.S.M.; Rodrigues,L.; Correa Junior,A.; Gautier,M.; Langella,P.; Azevedo,V.A.; Le Loir,Y.. |
| Lactococcus lactis, the model lactic acid bacterium, is a good candidate for heterologous protein production in both foodstuffs and the digestive tract. We attempted to produce Streptomyces tendae antifungal protein 1 (Afp1) in L. lactis with the objective of constructing a strain able to limit fungal growth. Since Afp1 activity requires disulfide bond (DSB) formation and since intracellular redox conditions are reportedly unfavorable for DSB formation in prokaryotes, Afp1 was produced as a secreted form. An inducible expression-secretion system was used to drive Afp1 secretion by L. lactis; Afp1 was fused or not with LEISSTCDA, a synthetic propeptide (LEISS) that has been described to be a secretion enhancer. Production of Afp1 alone was not achieved, but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactococcus lactis; Antifungal protein; Secretion; Streptomyces tendae antifungal protein 1. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100004 |
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| Seridan,B.; Souza,M.R.; Nicoli,J.R.; Carmo,L.S.; Menezes,L.D.M.; Oliveira,D.L.S.; Andrade,E.H.P.. |
| Staphylococcus aureus foi inoculado em queijos produzidos de forma estéril em laboratório, juntamente com Lactobacillus rhamnosus e Lactococcus lactis, isolados de queijo de coalho artesanal e identificados por PCR-ARDRA16S-23S. L. lactis foi capaz de reduzir a contagem de S. aureus no primeiro dia após produção (P<0,05) dos queijos de 3,3x10(7)UFC/g para 1,0x10(7)UFC/g. L. rhamnosus não impediu o crescimento de S. aureus. A presença das cepas acidoláticas, principalmente L. lactis, mostrou ainda potencial de inibição da produção de enterotoxina estafilocócica do tipo B, sendo que a concentração de enterotoxinas no 15° dia foi inferior ao limite de detecção pelo kit comercial utilizado. Concluiu-se que a presença das bactérias acidoláticas estudadas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Queijo; Staphylococcus aureus; Lactococcus lactis; Lactobacillus rhamnosus; Enterotoxinas; Qualidade sanitária. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200029 |
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